(Image: Network Rail)
No trains will run between York and Leeds for nine days later this month.
The disruption will take place from Saturday (October 24) to Sunday (November 1) to allow major upgrade work on the Transpennine Route Upgrade (TRU) project in the Church Fenton area.
Direct rail replacement buses will operate between York and Leeds, with additional stopping services serving intermediate stations.
Adam Sellers, senior sponsor for TRU, said: "We’re now in the final phase of installing the new Common Lane bridge following two successful weekends of work in Church Fenton.
"This nine-day closure allows our teams to carry out the reconstruction safely and will take us a step closer to electrifying this section of the route.
"I’d like to thank both customers and our local communities for their patience during this essential work which will help us deliver greener, quieter, electrified journeys across the North."
The closure follows two weekends of work to remove the old Common Lane railway bridge, which dated back to 1900-1901.
Network Rail inspections suggested it would need replacing in 2027, and TRU is carrying out the replacement a year earlier as the current structure is too low to accommodate the overhead line equipment needed to power electrified trains across the North.
Carl Harland, TRU customer lead for Northern, said: "There never is an ideal time to close parts of the railway, however, the works taking place at Church Fenton is a crucial next step in unlocking future benefits that the TRU programme will deliver between York, Leeds, Huddersfield and Manchester.
"While work is taking place at Church Fenton, trains will not be able to run between Leeds and York via Garforth.
"A direct rail replacement bus will operate between the two cities and is the quickest way for customers to travel, while buses will also serve local stations we are unable to call at, connecting into rail services that are still running.
"Whilst we have worked hard to ensure the impact journeys is minimal, our advice to customers is that they should check their journey before they travel and to allow extra time for their travel.
"Our teams will be on hand to support customers during this time."
Customers travelling between Darlington, York and Doncaster will also face disruption, with buses replacing trains due to separate improvement works on the East Coast Main Line.