The 24 National League clubs join the competition for the fourth qualifying round
Ninth-tier SE Dons, the former Sunday league team created by rapper Don Strapzy, will travel to Sholing with a place in the first round of the FA Cup at stake.
SE Dons, from Bexleyheath in London, are in the Southern Counties East Premier Division in only their third season in non-league football.
Their fourth qualifying round opponents from Hampshire compete in tier seven in the Southern League Premier Division South.
The other ninth-tier side left in the FA Cup fourth qualifying round, Tadley Calleva, from just north of Basingstoke, are away to National League side Aldershot Town.
Matches will be played on and around the weekend of Saturday, 17 October, with winners of the 32 ties joining the League One and Two clubs in the competition proper in November.
Northern section
Harborough Town v Spennymoor Town
Chorley v Worcester City
Gateshead v Stafford Rangers or Halesowen Town
Halifax Town v South Shields
Leiston v Guiseley
Barrow v Gloucester City
Hartlepool United v Marine
Boston United v Scunthorpe United
Macclesfield or Scarborough Athletic v Anstey Nomads
Altrincham v FC United of Manchester
Bury FC v Harrogate Town
Bury Town or Spalding United v Alvechurch
Rushden & Diamonds v Morecambe
Kidderminster Harriers v Solihull Moors
Carlisle United v Tamworth
Fylde v Trafford or Atherton Collieries
Southern section
Salisbury v Eastbourne Borough
Sutton United v Truro City
Chesham United v Forest Green Rovers
Leatherhead v Yeovil Town
Bedford Town or Wingate & Finchley v Cray Wanderers
Uxbridge v Hornchurch
Aldershot Town v Tadley Calleva
Torquay United v Oxford City
Wimborne Town v Chelmsford City
Wealdstone v Worthing
Boreham Wood v Woking
Eastleigh v Brentwood Town
Southend United v Berkhamsted
Sholing v SE Dons
Plymouth Parkway v Chertsey Town
Dover Athletic v Dorking Wanderers
Matches will be played on and around the weekend of Saturday, 17 October.